WO2015168521A2 - P97-polynucleotide conjugates - Google Patents

A-level Biology/Biology Foundation/biological molecules

WOA2 en. In some embodiments, the antisense strand is su bstantially or fully complementary to a target mRNA sequence of a gene selected from Table 2. The base, for example, may comprise one or more of the following: For in vivo use, for instance, for the treatment of hu man disease, medical imaging, or testing, the p97 conjugates described herein are generally incorporated into a pharmaceutical composition prior to administration.

There is abundant evidence that NOX- deficient mice are protected from stroke. See, e.

As one example, recursive ensemble mutagenesis REM , a technique which enhances the frequency of functional mutants in the libraries, can be used in combination with the screening assays to identify polypeptide variants Arkin and Yourvan, PNAS USA This allowance for high pressures help provide support for the plant by making the tissues rigid and are responsible for cell expansion during growth. When the TTC staining was completed, infarct size was measured by a colorimetric staining method.

Also included are methods for the treatment of a disease in a subject in need thereof, comprising administering to the subject a p97 conjugate or pharmaceutical composition described herein. Yang et al.

Polysaccharide: Definition, Examples, Function and Structure Biology Dictionary

Notify of. Cellulose and amylose are structurally different, and amylase does not recognize cellulose. In certain embodiments, pharmaceutical compositions and preparations according to the present invention are prepared so that a parenteral dosage unit contains between 0. Italy - Italia. Figure 6 illustrates a reaction scheme for conjugating a p97 polypeptide to a polynucleotide strand of an siRNA molecule to create a psiRNA conjugate.


As one problem, the blood-brain barrier BBB blocks the free transfer of many agents from blood to brain. Spacers may include, but are not be limited to, alkyl groups, alkenyl groups, alkynyl groups, aryl groups, aralkyl groups, aralkenyl groups, aralkynyl groups; each of which can contain one or more heteroatoms, heterocycles, amino acids, nucleotides, and saccharides.

Oligonucleotide Synthesis N. Add itionally, the expected score for aligning a random pair of amino acids is required to be negative, otherwise long alignments would tend to have high score independently of whether the segments aligned were related.

In certain aspects, the p97 polypeptide sequence and the siRNA molecule s are each, individually or as a pre-existing conjugate, bound to or encapsulated within a particle, e. In some embodiments, from 1- 4 or from 5 to 15 of the C atoms are substituted with a heteroatom independently selected from O, N, S. By "isolated" is meant material that is substantially or essentially free from components that normally accompany it in its native state.

A-level Biology/Biology Foundation/biological molecules - Wikibooks, open books for an open world

It may be possible to exploit RNA interference in therapy. Exemplary markers of clinical improvement will be apparent to persons skilled in the art. The terms "PEG," "polyethylene glycol" and "poly ethylene glycol " as used herein, are interchangeable and meant to encompass any water-soluble poly ethylene oxide derivative. El Salvador.

WOA2 - Ppolynucleotide conjugates - Google Patents

The composition for rectal administration may contain an oleaginous base as a suitable nonirritating excipient. The choice of gap costs, G and L is empirical, but it is customary to choose a high value for G , e. A "comparison window" refers to a conceptual segment of at least 6 contiguous positions, usually a bout 50 to a bout , more usually a bout to a bout in which a sequence is compared to a reference sequence of the same nu mber of contiguous positions after the two sequences are optimally aligned.

Synthetic Biology: If intended for transdermal administration, the composition may include a transdermal patch or iontophoresis device.